You Searched For: Sanger\'s+reagent

9 169 results were found

Refine Results Sort by

Sort Results

List View Easy View (new)

Compare(0) Clear

Rate These Search Results

ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] 10 mM in ddH₂O (1 µmole)

Catalog Number: (AATB17210)

Supplier: AAT BIOQUEST

Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

UOM: 1 * 1 µmol

Sale

N/A Each

ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] 10 mM in ddH₂O (1 µmole)

Catalog Number: (AATB17209)

Supplier: AAT BIOQUEST

UOM: 1 * 1 µmol

Sale

N/A Each

Axygen® AxyPrep® Mag DyeClean kit

Supplier: Corning

Description: Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.

PCR clean-up kit, Axygen® AxyPrep™ Mag

Supplier: Corning

Description: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Genomic DNA extraction from dried blood spots, Extracta™ DBS

Supplier: Quantabio

Description: Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

MSDS

Aminopropargyl ddCTP for Sanger sequencing

Catalog Number: (AATB17070)

Supplier: AAT BIOQUEST

Description: Sanger method is one of the most reliable and earliest DNA sequencing methods.

UOM: 1 * 10 µmol

Sale

N/A Each

Aminopropargyl ddTTP for Sanger sequencing

Catalog Number: (AATB17072)

Supplier: AAT BIOQUEST

Description: Sanger method is one of the most reliable and earliest DNA sequencing methods.

UOM: 1 * 10 µmol

Sale

N/A Each

Aminopropargyl ddATP for Sanger sequencing

Catalog Number: (AATB17074)

Supplier: AAT BIOQUEST

Description: Sanger method is one of the most reliable and earliest DNA sequencing methods.

UOM: 1 * 10 µmol

Sale

N/A Each

Aminopropargyl ddGTP for Sanger sequencing

Catalog Number: (AATB17076)

Supplier: AAT BIOQUEST

Description: Sanger method is one of the most reliable and earliest DNA sequencing methods.

UOM: 1 * 10 µmol

Sale

N/A Each

Peroxidase Indicator Reagent

Catalog Number: (3901-10VL)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.

UOM: 1 * 10 items

Sale