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Product Image-732-3267

Peqlab, Mid Range and Long Range PCR Systems

Catalog Number: (732-3267)
Supplier: VWR Peqlab
Description: PCR products of up to 12 kb and up to 40 kb can be achieved with the high performance peqGOLD 'Mid Range' and 'Long Range' PCR Systems respectively, suitable for both genomic and episomal templates. Applicable in place of standard <i>Taq</i> DNA polymerase for PCR amplifications when longer fragments and larger amounts are desirable, including sequencing, mapping and λ-phage or cosmid studies.
UOM: 1 * 1 items

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Image Unavailable-ENZOENZ42731

Bio-16-ddUTP dideoxynucleotide pack for 3’-oligonucleotide labeling

Catalog Number: (ENZOENZ42731)
Supplier: ENZO LIFE SCIENCES
Description: The procedure for labeling of DNA probes with a polynucleotide 'tail' containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides.
UOM: 1 * 1 KIT

New Product


Image Unavailable-SOLUL-8106-100

TriLink® CleanCap® M6 Cas9 mRNA (N1MePsU)

Supplier: TriLink BioTechnologies
Description: <p>Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes </em>SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double-stranded DNA cleavage.</p>

New Product

Product Image-733-2553

VWR®, TEMPase Hot Start DNA polymerase

Supplier: VWR Chemicals
Description: VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR. A glycerol-free TEMPase Hot Start DNA Polymerase is also available for automation and freeze drying.

Image Unavailable-SOLUL-7206-5

TriLink® CleanCap® Cas9 mRNA (5 moU)

Supplier: TriLink BioTechnologies
Description: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest, and the Cas9 protein is employed to perform double-stranded DNA cleavage.

Product Image-ENZOENZ42501

Cyanine 3-dUTP

Catalog Number: (ENZOENZ42501)
Supplier: ENZO LIFE SCIENCES
Description: Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.
UOM: 1 * 25 Nmo

New Product


Product Image-733-2106

AccuStart™ Genotyping ToughMix®

Supplier: Quantabio
Description: AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

Certificates

Product Image-733-2265

PerfeCTa® PreAmp 5X SuperMix

Catalog Number: (733-2265)
Supplier: Quantabio
Description: PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.
UOM: 1 * 40 Assays
Image Unavailable-ENZOENZ42502

Cyanine 5-dUTP

Catalog Number: (ENZOENZ42502)
Supplier: ENZO LIFE SCIENCES
Description: Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.
UOM: 1 * 25 Nmo

New Product


Product Image-BSENR-1312-100

Anti-His Tag Rabbit Polyclonal Antibody

Catalog Number: (BSENR-1312-100)
Supplier: Biosensis
Description: The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
UOM: 1 * 100 µG
Image Unavailable-733-1273

qPCR SuperMix, PerfeCTa® MultiPlex

Supplier: Quantabio
Description: This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.

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Product Image-BSENM-1311-100

Anti-His Tag Mouse Monoclonal Antibody [clone: BHISH8]

Catalog Number: (BSENM-1311-100)
Supplier: Biosensis
Description: The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
UOM: 1 * 100 µG
Image Unavailable-BOSSBS-5364R

Anti-HSF1 Rabbit Polyclonal Antibody

Catalog Number: (BOSSBS-5364R)
Supplier: Bioss
Description: Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.
UOM: 1 * 100 µl
Product Image-25-9005-98

Sequencing reaction purification, illustra™ AutoScreen 96 Well Plates

Catalog Number: (25-9005-98)
Supplier: Cytiva
Description: AutoScreen 96 well plate consists of a 96-well filter plate containing DNA grade Sephadex™ G-50 for purification of sequencing reactions prior to analysis on MegaBACE 1000 and can be used for other size exclusion applications.
UOM: 1 * 10 items
Image Unavailable-786-14

Lysine-C™, sequencing grade

Catalog Number: (786-14)
Supplier: G-Biosciences
Description: SG-Lysine-C™ endopeptidase, from <i>Lysobacter enzymogenes</i>, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.
UOM: 1 * 2 items
Image Unavailable-786-13

Chymotrypsin™, sequencing grade

Catalog Number: (786-13)
Supplier: G-Biosciences
Description: SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
UOM: 1 * 2 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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