You Searched For: Sanger+Sequencing+Reagents


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Catalog Number: (786-13)
Supplier: G-Biosciences
Description: SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
UOM: 1 * 2 items


Supplier: Cytiva
Description: For the amplification of difficult templates for successful DNA sequencing in 18 hours.

Catalog Number: (786-11)
Supplier: G-Biosciences
Description: SG-Arginine-C™ endopeptidase (Clostripain, from <i>C. histolyticum</i>) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
UOM: 1 * 2 items


Catalog Number: (25-6400-80)
Supplier: Cytiva
Description: illustra™ TempliPhi large construct DNA amplification kit (TempliPhi LC kit) is used to prepare templates for BAC or fosmid DNA sequencing.
UOM: 1 * 1.000 Tests


Catalog Number: (786-15)
Supplier: G-Biosciences
Description: SG-Glutamic-C™ is a serine endopeptidase, from<i> S. aureus V8</i>, that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is specific for the glutamyl site. Recommended buffers for fragmentation of proteins using this enzyme are 50 mM Tris-HCI, pH 8,0 or bicarbonate buffer. Highly purified preparations of SG-Glutamic-C™ are chemically modified making the enzyme both resistant to autolysis and stabilises its enzymatic activity.
UOM: 1 * 2 items


Supplier: AAT BIOQUEST
Description: 3'-O-Azidomethyl-dATP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

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Supplier: AAT BIOQUEST
Description: 3'-O-Azidomethyl-dTTP is one of the four essential dNTPs (A, C, G, T) that are used for the DNA sequencing by synthesis (SBS).

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Catalog Number: (AATB17035)
Supplier: AAT BIOQUEST
Description: Fluorescein-12-dCTP might be incorporated into a DNA sequence as its natural counterpart dCTP to incorporate a fluorescein tag in a DNA molecule.
UOM: 1 * 25 Nmo

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Supplier: AAT BIOQUEST
Description: 3'-O-Azidomethyl-dGTP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

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Catalog Number: (STMC79003)
Supplier: STEMCELL Technologies
Description: For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing.
UOM: 1 * 1 KIT


Supplier: Cytiva
Description: dNTPs designed for applications requiring high purity such as amplification, dideoxy sequencing, labeling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: AAT BIOQUEST
Description: 2'-Deoxycytidine-5'-Triphosphate (dCTP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

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Supplier: Merck
Description: The Montage® <sub>SEQ96</sub> sequencing reaction cleanup kit provides the filtration plate and solution necessary to remove contaminating salts and unincorporated dye terminators from DNA sequencing reactions.

Supplier: Cytiva
Description: PCR nucleotide mix dNTP set (2 mM, 10 mM, or 25 mM each A,C,G,T) for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Catalog Number: (27-2090-02)
Supplier: Cytiva
Description: Band compressions are ambiguous band patterns on sequencing gels caused by stable intra-strand secondary structures that are not fully denatured during electrophoresis. Their occurrence can be reduced by substituting nucleotide analogues in the sequencing mixes. 7-Deaza dGTP (c⁷dGTP) can be used to resolve G-band compressions.
UOM: 1 * 2 µmol


Supplier: AAT BIOQUEST
Description: 3'-O-Azidomethyl-dCTP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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