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Product Image-PIER22360

SMCC (N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate), Pierce™

Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
Catalog Number: PIER22360
UOM: 1 * 50 mg
Supplier: Thermo Fisher Scientific

MSDS Certificates


Product Image-PIER26777

Sulphosuccinimidyl acetate (Sulpho-NHS-acetate), Pierce™

Description: Pierce™ Sulfo-NHS-Acetate is a small compound used for blocking primary amines (eg. lysine side chains). This compound forms stable, covalent amide bonds with amine groups to prevent specific interactions or conjugations.
Catalog Number: PIER26777
UOM: 1 * 100 mg
Supplier: Thermo Fisher Scientific
Image Unavailable-PIER15162

NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™

Description: QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
Catalog Number: PIER15162
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-PIER17926

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Description: Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
Catalog Number: PIER17926
UOM: 1 * 1 kg
Supplier: Thermo Fisher Scientific
Product Image-PIER22301

PDPH ((3-(2-pyridyldithio)propionyl hydrazide)), Pierce™

Description: Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Catalog Number: PIER22301
UOM: 1 * 50 mg
Supplier: Thermo Fisher Scientific
Product Image-PIER21857

SPDP (N-Succinimidyl-3-(2-pyridyldithio)propionate), Pierce™

Description: Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
Catalog Number: PIER21857
UOM: 1 * 50 mg
Supplier: Thermo Fisher Scientific

Certificates


Product Image-PIER22589

Sulpho-SANPAH (Sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), Pierce™

Description: Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.
Catalog Number: PIER22589
UOM: 1 * 50 mg
Supplier: Thermo Fisher Scientific
Product Image-PIER21651

LC-SPDP (Succinimidyl 6-[3-(2-pyridylthio)propionamidohexanoate), Pierce™

Description: Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
Catalog Number: PIER21651
UOM: 1 * 50 mg
Supplier: Thermo Fisher Scientific
Product Image-PIER21650

Sulpho-LC-SPDP (Sulphosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate) ≥90%, Pierce™

Description: Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.
Catalog Number: PIER21650
UOM: 1 * 50 mg
Supplier: Thermo Fisher Scientific
Product Image-PIER28100

PMPI (4-Maleimidophenyl isocyanate), Pierce™

Description: Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.
Catalog Number: PIER28100
UOM: 1 * 50 mg
Supplier: Thermo Fisher Scientific
Product Image-PIER34000

CN/DAB substrate kit, Pierce™

Description: Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
Catalog Number: PIER34000
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-PIER34070

Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor

Description: The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and <i>in situ</i> hybridisation applications.
Catalog Number: PIER34070
UOM: 1 * 100 mL
Supplier: Thermo Fisher Scientific
Product Image-PIER44918

Pierce™ Antibody Purification Kits, Chicken IgY Purification Kit

Description: Pierce™ chicken IgY purification kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.
Catalog Number: PIER44918
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Image Unavailable-PIER77016

Blotting membranes, nylon, Biodyne™ B

Description: Biodyne™ B nylon membranes are sheets of positively charged nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. Biodyne™ B nylon membrane is manufactured with a matrix pore size of 0,4 micron. It contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules, like DNA and RNA.
Catalog Number: PIER77016
UOM: 1 * 25 items
Supplier: Thermo Fisher Scientific
Image Unavailable-PIER24582

Pierce™ Zinc Reversible Stain Kit

Description: Thermo Scientific Pierce zinc reversible stain kit provides rapid and highly sensitive detection of protein in polyacrylamide gels immediately after electrophoresis as well as erasability for subsequent transfer or band extraction.
Catalog Number: PIER24582
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-PIER78610

Protein A/G magnetic agarose beads, Pierce™

Description: Protein A/G magnetic agarose beads provide a fast, convenient method for purification of immunoglobulins from serum, cell culture supernatant, or ascites.
Catalog Number: PIER78610
UOM: 1 * 20 mL
Supplier: Thermo Fisher Scientific
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