You Searched For: Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Description: GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Catalog Number: (EN0771)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA, such as cDNA and primers are not cleaved. dsDNase is easily inactivated by moderate heat treatment (55 °C). These features make dsDNase an excellent choice for gDNA elimination prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

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Catalog Number: (R0641)

Supplier: Thermo Fisher Scientific

Description: In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA. RNA loading dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilises RNA.

UOM: 1 * 1 KIT

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Catalog Number: (R1181)

Supplier: Thermo Fisher Scientific

Description: Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Catalog Number: (SM0271)

Supplier: Thermo Fisher Scientific

Description: pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

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Supplier: Thermo Fisher Scientific

Description: This nuclease-free water is deionized and 0.22 µM membrane-filtered nuclease-free water

Supplier: Thermo Fisher Scientific

Description: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Supplier: Thermo Fisher Scientific

Description: Exonuclease I (ExoI) degrades single-stranded DNA in a 3' to 5'direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

Catalog Number: (EO0461)

Supplier: Thermo Fisher Scientific

Description: Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase

Catalog Number: (R0862)

Supplier: Thermo Fisher Scientific

Description: Dithiothreitol (DTT, Cleland's reagent)

UOM: 1 * 1 KIT

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Catalog Number: (ER1921)

Supplier: Thermo Fisher Scientific

Description: CCWGG sites and cuts best at 37 °C in O buffer.

UOM: 1 * 1 KIT

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