You Searched For: G Bioscienes

Catalog Number: (786-182)

Supplier: G-Biosciences

Description: The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

Supplier: G-Biosciences

Description: Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, <i>E.coli</i>, yeast, plant or as a control set that contains one 2 mg vial of each.

Catalog Number: (R034)

Supplier: G-Biosciences

Description: Ethidium bromide 10 mg/ml in aqueous solution

UOM: 1 * 10 mL

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Supplier: G-Biosciences

Description: N-Decyl-N,N-dimethyl-3-ammonio-1-propanesulphonate(sulphobetaine-10, SB-10)

Supplier: G-Biosciences

Description: Nonidet® P 40 Substitute (NP-40) 10% in aqueous solution

Supplier: G-Biosciences

Description: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Catalog Number: (786-231)

Supplier: G-Biosciences

Description: The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.

Catalog Number: (786-241)

Supplier: G-Biosciences

Description: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

UOM: 1 * 1 KIT

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Catalog Number: (786-250)

Supplier: G-Biosciences

Description: Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Catalog Number: (RC-030)

Supplier: G-Biosciences

Description: Calcium chloride dihydrate

UOM: 1 * 500 g

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Catalog Number: (786-224)

Supplier: G-Biosciences

Description: Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.

UOM: 1 * 50 mL

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Catalog Number: (786-226)

Supplier: G-Biosciences

Description: Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimise reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on 2D gels.

UOM: 1 * 50 mL

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Supplier: G-Biosciences

Description: Protein assay, Resorufin-Casein protease substrate