You Searched For: tert-Butanol

Description: 'NO-LOSS' dialyser.

Catalog Number: 514-0160

UOM: 1 * 20 items

Supplier: G-Biosciences

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Description: Protein assay, Bovine γ-Globulin Protein Standard (2 mg/ml)

Catalog Number: 786-006

UOM: 1 * 2 items

Supplier: G-Biosciences

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Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Catalog Number: SLR-027

UOM: 1 * 150 µG

Supplier: G-Biosciences

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Description: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.

Catalog Number: 786-022

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.

Catalog Number: 786-651

UOM: 1 * 500 Assays

Supplier: G-Biosciences

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Description: For improved dialysis time and rates.

Catalog Number: 786-627

UOM: 1 * 50 mL

Supplier: G-Biosciences

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Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Catalog Number: 786-252

UOM: 1 * 50 Tests

Supplier: G-Biosciences

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Description: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

Catalog Number: 786-680

UOM: 1 * 50 mL

Supplier: G-Biosciences

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Description: Swift™ Film Cleaner allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. Suitable for all exposed film developed for gel shift assays, Western, Southern and Northern blots.

Catalog Number: 786-678

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Catalog Number: 786-528

UOM: 1 * 1 L

Supplier: G-Biosciences

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Description: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Catalog Number: BE-401

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: EDTA disodium salt is a reversible metalloprotease inhibitor that is used for chelating metal ions.

Catalog Number: 786-050

UOM: 1 * 100 g

Supplier: G-Biosciences

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Description: A proprietary mixture of Formaldehyde, EDTA and Blue Dextran that is designed for use in loading DNA samples onto denaturing polyacrylamide gels.

Catalog Number: 786-556

UOM: 1 * 1 items

Supplier: G-Biosciences

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Description: Ready to screen human cancer cell line blots.

Catalog Number: TB55

UOM: 1 * 1 items

Supplier: G-Biosciences

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Description: These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.

Catalog Number: TB45

UOM: 1 * 1 items

Supplier: G-Biosciences

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Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Catalog Number: TLH-01

UOM: 1 * 150 µG

Supplier: G-Biosciences

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