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Supplier: Thermo Fisher Scientific
Description: The Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kits use immobilised ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments.

Catalog Number: (PIER44688)
Supplier: Thermo Fisher Scientific
Description: Pierce™ F(ab')2 micro preparation kit enables efficient Fab generation from IgG.
UOM: 1 * 1 KIT


Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.
Supplier: Thermo Fisher Scientific
Description: These Ni(2+) chelate-coated plates are ideal for analysing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.
Supplier: Thermo Fisher Scientific
Description: This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Supplier: Thermo Fisher Scientific
Description: Pierce

Supplier: Thermo Fisher Scientific
Description: Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Supplier: Thermo Fisher Scientific
Description: The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

Supplier: Thermo Fisher Scientific
Description: Desthiobiotin-ATP probe, 16×12,6 µg

Supplier: Thermo Fisher Scientific
Description: The Active GTPase Pull-Down and Detection Kits allows the monitoring of changes in GTPase activity levels by enriching and detecting the active (GTP-bound) state of a particular GTPase.

Catalog Number: (PIER28300)
Supplier: Thermo Fisher Scientific
Description: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)
UOM: 1 * 5 g


Supplier: Thermo Fisher Scientific
Description: Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.

Catalog Number: (PIER37615)
Supplier: Thermo Fisher Scientific
Description: The 1-Step™ ABTS (2,2'-azinobis [3-ethylbenzthiazoline-6-sulfonic acid] diammonium salt) is a ready-to-use, water-soluble ELISA substrate for horseradish peroxidase. This substrate yields a green product with absorbance maxima at 410 nm and 650 nm. Adding SDS to the substrate stops the reaction without changing the colour.
UOM: 1 * 250 mL


Catalog Number: (PIER32400)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
UOM: 1 * 1 mg

MSDS


Supplier: Thermo Fisher Scientific
Description: Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilise proteins, glycoproteins, or nucleic acids. Protein immobilisation is thought to occur by hydrophobic interactions, and high salt and low methanol concentrations help improve protein immobilisation to the membrane during electrophoretic transfer, especially for proteins with higher molecular weights. Nitrocellulose membranes are not optimal for electrophoretic transfer of nucleic acids, as the high salt concentrations that are required for efficient binding will effectively elute some or all of the charged nucleic acid fragments.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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