You Searched For: Educational Materials

Catalog Number: (EDVO957)

Supplier: EDVOTEK

Description: According to the World Health Organisation, between two and three million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

UOM: 1 * 1 KIT

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Catalog Number: (EDVO990)

Supplier: EDVOTEK

Description: When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.

UOM: 1 * 1 items

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Supplier: EDVOTEK

Description: This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

Catalog Number: (EDVO956)

Supplier: EDVOTEK

Description: Oil spills cause devastation to the environment, killing sea life, birds, and coastal plants. Spraying areas of contamination with oil-eating microbes accelerates the degradation of the oil. This process is known as bioremediation. In this open-ended experiment, students grow a mixture of oil-eating bacteria and observe their effectiveness at degrading a variety of oils.

UOM: 1 * 1 KIT

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Catalog Number: (763-0002)

Supplier: EDVOTEK

Description: This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

UOM: 1 * 1 KIT

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Catalog Number: (EDVO222)

Supplier: EDVOTEK

Description: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.

UOM: 1 * 1 items

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Catalog Number: (EDVO255)

Supplier: EDVOTEK

Description: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.

UOM: 1 * 1 KIT

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Catalog Number: (EDVOS-70)

Supplier: EDVOTEK

Description: The body defends itself from attack by infectious agents like bacteria and viruses by producing antibodies. Enzyme linked immunosorbent assays (ELISA) test for antibodies present in the blood, which indicate infection. In this experiment, students perform a simulated ELISA test to identify infected samples and compare them to control samples.

UOM: 1 * 1 items

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Catalog Number: (EDVO141)

Supplier: EDVOTEK

Description: Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.

UOM: 1 * 1 items

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Catalog Number: (EDVO303)

Supplier: EDVOTEK

Description: Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

UOM: 1 * 1 KIT

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Catalog Number: (EDVO166)

Supplier: EDVOTEK

Description: An infectious outbreak requires prompt and accurate identification of the biological agent. Often, early clinical symptoms are first identified in exposed individuals and then infectious agents are identified by lab tests. In this experiment, students transmit a simulated infectious agent (chemical dye) between classmates which is only visible under long UV light. The pattern of transmission and primary source is documented.

UOM: 1 * 1 items

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Supplier: EDVOTEK

Description: The three most frequently used restriction enzymes are Eco RI, Bam HI and Hind III. Each enzyme catalyses cleavage at the defined base sequence. All enzymes are lyophilised and contain 1500 units. One unit is defined as the amount of enzyme required to digest 1,0 µg of lambda DNA in 60 minutes at 37 °C in a total reaction mixture of 50 µl.

Supplier: EDVOTEK

Description: DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

Supplier: EDVOTEK

Description: In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.

Catalog Number: (EDVOS-91)

Supplier: EDVOTEK

Description: Evidence left behind at a crime scene can identify a potential culprit. Even in this age of DNA, fingerprints and blood stains are still important in helping to identify a criminal. In this experiment, students solve a crime by dusting for fingerprints and use fluorescent dust to search for and identify trace amounts of blood.

UOM: 1 * 1 items

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Catalog Number: (763-0010)

Supplier: EDVOTEK

Description: This simple experiment demonstrates detection of the mutation that causes Sickle Cell Anaemia. In this simulation, students use electrophoresis to separate dyes that represent patient samples and controls.

UOM: 1 * 1 items

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