Rhod-2 AM (Bromide) fluorescent indicator, for calcium determination
Supplier: STEMCELL Technologies
A cell-permeable calcium dye that exhibits fluorescence upon increasing Ca2+ concentrations.
- Excitation wavelength: 553 nm
- Emission wavelength: 577 nm
- Dissociation constant (Kd) = 570 nM
- Verified for use in fluorescence microscopy
Rhod-2 AM is a cell-permeable acetoxymethyl (AM) ester dye, which fluoresces in the presence of Ca2+. Cellular uptake of Rhod-2 AM is facilitated by the AM group, which is removed by cytoplasmic esterases, resulting in intracellular accumulation (Smith et al. Sci Signal, 2018). Rhod-2 AM selectively accumulates in the mitochondria. Increasing Ca2+ concentrations significantly enhance Rhod-2 AM fluorescence. The long emission wavelengths of this dye make it valuable for applications involving cells that have high levels of autofluorescence.
Increased intracellular calcium concentrations are a critical indicator of activation of apoptotic pathways. (Mukherjee et al. J Biol Chem, 2002). Use Rhod-2 AM (Bromide) dye to measure mitochondrial calcium influx by detection using flow cytometry, fluorescence microscopy, and microplate readers.
Upozornení: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').
Formula:
C₅₂H₅₉BrN₄O₁₉ MW: 1124,0 g/mol |
CAS Number:
145037-81-6 |
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