You Searched For: STEMCELL Technologies

Catalog Number: (STMC07820)

Supplier: STEMCELL Technologies

Description: Density gradient medium for the isolation of viruses, organelles, macromolecules, or cells.

UOM: 1 * 250 mL

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Catalog Number: (STMC10955)

Supplier: STEMCELL Technologies

Description: Supplement for the differentiation of mouse naïve CD4+ T cells into Th2 cells.

UOM: 1 * 1 mL

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Catalog Number: (STMC10973)

Supplier: STEMCELL Technologies

Description: Culture supplement for the differentiation of human naïve CD4+ T cells into Th1 cells.

UOM: 1 * 1 mL

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Catalog Number: (STMC36253)

Supplier: STEMCELL Technologies

Description: Dulbecco's Modified Eagle's Medium (DMEM) with 1000 mg/L D-glucose.

UOM: 1 * 500 mL

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Supplier: STEMCELL Technologies

Description: Receptor activator of nuclear factor kappa-B ligand (RANKL) is a member of the tumor necrosis factor (TNF) superfamily (Anderson <i>et al.</i>). Cytokines in the TNF superfamily are involved in a variety of long-term cellular activities such as differentiation, proliferation, and cell death (MacEwan). RANKL is a type II homotrimeric transmembrane protein expressed in both a membrane-bound and secreted form (Ikeda <i>et al.</i>). RANKL binds to the receptor activator of nuclear factor kappa-B (RANK). Upon binding to its receptor, RANKL activates the AKT signaling pathway (Moon <i>et al.</i>). Osteoprotegerin (OPG) may also bind RANKL, and this binding competes with RANKL-RANK binding (Lacey <i>et al.</i>). RANKL is involved in osteoclastogenesis (Lacey <i>et al.</i>; Yasuda <i>et al.</i>) and T cell activation (Wong <i>et al.</i>).

Supplier: STEMCELL Technologies

Description: Interleukin 13 (IL-13) is a cytokine important in type 2 immune responses and is expressed by T helper type 2 (Th2) cells and group 2 innate lymphoid cells (ILC2s) (Pulendran and Artis). IL-13 binds a receptor composed of IL-4Ra and IL-13Ra1 or IL-13Ra2 (Wynn 2003). IL-13 receptor is expressed on B cells and promotes B cell proliferation, induces class switching to IgG4 and IgE, and functions in the recruitment and activation of IgE-producing B cells (Hershey). The receptor is also expressed on basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells (Hershey). Signaling through the IL-13 receptor activates the JAK/STAT and IRS-1/IRS-2 pathways. <i>in vivo</i>, IL-13 has a role in resistance to extracellular helminth parasites by regulating gastrointestinal parasite expulsion, as well as in airway hyperresponsiveness, allergic inflammation, tissue remodeling, tumor cell growth, and fibrosis (Wynn 2015). Secreted IL-13 is a protein consisting of 112 amino acids with a molecular mass of 10 kDa (Hershey). Human IL-13 is not species-specific but has greater activity on human cells compared to mouse cells (Hershey).

Catalog Number: (STMC75002)

Supplier: STEMCELL Technologies

Description: Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases.

UOM: 1 * 10 mg

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Supplier: STEMCELL Technologies

Description: Fibroblast growth factor 18 (FGF-18) is a growth factor and member of the FGF subfamily. FGF-18 is most similar to FGF-8 and FGF-17, and is secreted in adult lung and developing tissues (Ohbayashi <i>et al.</i>). FGF-18 signals through FGF receptor 3 (FGFR3) to regulate proliferation, differentiation, and matrix production of articular and growth plate chondrocytes <i>in vivo</i> and in vitro (Davidson <i>et al.</i>). FGF-18 has skeletal functions and protects articular cartilage by gene expression profiling and regulates proliferation and differentiation of midline cerebellar structures (Mori <i>et al.</i>). Also, recombinant human FGF-18 has been shown to effectively regulate hair growth (Song <i>et al.</i>).

Supplier: STEMCELL Technologies

Description: Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto <i>et al.</i>; Westermark and Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src and PI3K/AKT (Kim <i>et al.</i>). PDGF is a potent mitogen for cells of mesenchymal origin- like fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto <i>et al.</i>; Sachinidis <i>et al.</i>). PDGF-BB is secreted by osteoblasts to induce mesenchymal stem cell migration and angiogenesis. It has also been shown that PDGF-BB is secreted by preosteoclasts during bone modeling and remodeling to induce angiogenesis and thus proper osteogenesis (Xie <i>et al.</i>).

Supplier: STEMCELL Technologies

Description: Complete kit for optimisation of genome editing in human cells.

Catalog Number: (STMC100-1282)

Supplier: STEMCELL Technologies

Description: Supplement (50X) without vitamin A for the culture of neural progenitor cells.

UOM: 1 * 100 mL

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Catalog Number: (STMC100-1388)

Supplier: STEMCELL Technologies

Description: Stimulating T cells with EBV (LMP1) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (LMP1) Peptide pool is a lyophilised mixture of 94 peptides from latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV; strain B95-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 386 on LMP1. LMP1 is essential for EBV-mediated growth transformation of B cells (Kaye <i>et al.</i>) and has been shown to downregulate toll-like receptor 9 (TLR9) expression in infected cells, a mechanism the virus may use to escape host innate immune recognition (Fathallah <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

UOM: 1 * 1 AU

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Catalog Number: (STMC100-1403)

Supplier: STEMCELL Technologies

Description: Stimulating T cells with HHV8 (K8) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV8 (K8) peptide pool is a lyophilised mixture of 57 peptides from protein K8 alpha of human herpesvirus 8 (HHV-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 237 on K8. A basic leucine zipper (bZip) protein that associates with the lytic origin of HHV8 (Lin <i>et al.</i>), K8 is thought to play a role in lytic DNA replication, as a trans-acting factor (AuCoin <i>et al.</i>). It has also been found to modulate the activity of the transcriptional transactivator K-Rta (Izumiya <i>et al.</i>), and has an autoregulatory function (Wang <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

UOM: 1 * 1 AU

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Supplier: STEMCELL Technologies

Description: SARS-CoV-2 Recombinant Spike Protein, aa16-685 is expressed in HEK293 cells and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Spike Protein plays a key role in attachment to host cells, allowing invasion through clathrin-mediated endocytosis. The Spike Protein can be cleaved by host cell proteases after aa685 to yield the N-terminal S1 subunit and C-terminal S2 region. The S1 subunit is responsible for interacting with the host cell receptor (angiotensin-converting enzyme II) through a receptor-binding domain that is highly conserved with SARS-CoV. The S1 subunit has two conformations: a ‘down’ conformation in which the receptor is inaccessible, and an ‘up’ conformation in which the receptor is accessible. These conformational changes are key for monoclonal antibody drugs and vaccine development. SARS-CoV-2 Recombinant Spike Protein contains a polyhistidine tag at the amino terminus; it also contains a FLAG tag at the carboxy terminus.

Supplier: STEMCELL Technologies

Description: Brain-derived neurotrophic factor (BDNF), like nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4), is a member of the NGF family of neurotrophins, which are required for the differentiation and survival of specific neuronal subpopulations in both the central and the peripheral nervous systems (Minichiello and Klein; Minichiello <i>et al.</i>). BDNF binds with high affinity to the tropomyosin receptor kinase B (TrkB), and activates AKT and ERK pathways (Mattson <i>et al.</i>). It is expressed in the hippocampus, cortex, and synapses of the basal forebrain. BDNF acts as a survival factor for human embryonic stem cells when plated on either feeder cells or Corning® Matrigel® (Pyle <i>et al.</i>). BDNF regulates synaptic transmission and plasticity at adult synapses in the central nervous system, and contributes to adaptive neuronal responses including long-term potentiation, long-term depression, certain forms of short-term synaptic plasticity, and homeostatic regulation of neuronal excitability (Reichardt). It also has a role in neurogenesis by promoting survival and growth of dorsal root ganglion cells, and hippocampal and cortical neurons (Binder and Scharfman). BDNF, together with glial cell line-derived neurotrophic factor (GDNF) and other supplements, is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Brafman).

Catalog Number: (STMC100-0646)

Supplier: STEMCELL Technologies

Description: Serum-free and xeno-free base medium for the expansion of B cells.

UOM: 1 * 100 mL

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