You Searched For: 3-Indolyl-\u00DF-D-galactopyranoside

Catalog Number: (EDVO614)

Supplier: EDVOTEK

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

UOM: 1 * 250 mg

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Supplier: Merck

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside), Sigma-Aldrich®

Catalog Number: (MDTC46-101-RF)

Supplier: Corning

Description: X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) is a substrate of b-galactosidase, a gene product of the lac operon in <i>E. coli</i>.

UOM: 1 * 1 g

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Supplier: G-Biosciences

Description: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Supplier: Thermo Fisher Scientific

Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥98%

MSDS

Supplier: MP Biomedicals

Description: 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.

Catalog Number: (OZBIGX10003)

Supplier: OZ BIOSCIENCES

Description: X-Gal staining kit allows to visualise ß-gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates.

UOM: 1 * 1 KIT

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Supplier: Apollo Scientific

Description: 3-Indolyl butyrate

Supplier: Apollo Scientific

Description: (4-Nitrophenyl)-β-D-galactopyranoside

Catalog Number: (APOSBIB6104-25G)

Supplier: Apollo Scientific

Description: Methyl-α-D-galactopyranoside

UOM: 1 * 25 g

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Supplier: Apollo Scientific

Description: 2-Nitrophenyl α-D-galactopyranoside ≥98%

Supplier: Apollo Scientific

Description: 3-Nitrophenyl α-D-galactopyranoside ≥98%

Supplier: MP Biomedicals

Description: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Supplier: Apollo Scientific

Description: (4-Nitrophenyl)-α-D-galactopyranoside

Supplier: Thermo Fisher Scientific

Description: (4-Nitrophenyl)-β-D-galactopyranoside ≥98%

MSDS Certificates

Catalog Number: (A1272.0025)

Supplier: PanReac AppliChem

Description: (2-Nitrophenyl)-β-D-galactopyranoside (ONPG)

UOM: 1 * 25 g

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