You Searched For: Acridinium+C2+NHS+Ester

Catalog Number: (PIER21650)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

UOM: 1 * 50 mg

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

Catalog Number: (PIERA39268)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

UOM: 1 * 20 mg

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Catalog Number: (SIAL49667-50MG-F)

Supplier: Merck

Description: SPB is an amine-reactive and photoreactive heterobifunctional crosslinking reagent. The photoreactive psoralen group provides much greater covalent insertion yields than phenyl azide-containing compounds, which makes this reagent a superior alternative to typical photoreactive NHS-ester crosslinkers. The psoralen tricyclic planar ring system intercalates into double-stranded, and to a lesser extent, single-stranded, DNA, RNA, PCR products, cDNA or oligonucleotides. It also can be used with agarose gel-purified oligonucleotides because the presence of agarose does not interfere with the coupling reaction. The nucleic acid labeling site of the psoralen group (the 5,6 double bond in thymine residues) does not interfere with subsequent hybridization reactions, creating a highly-sensitive direct labeling alternative to radioactive probes.

UOM: 1 * 50 mg

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Catalog Number: (SIAL803421-50MG)

Supplier: Merck

Description: Succinimidyl-diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions, and these SDA reagents extend the efficiency and range of interactions that can be explored by this approach.

UOM: 1 * 50 mg

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Supplier: MP Biomedicals

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Catalog Number: (ENZOBMLSL1010005)

Supplier: ENZO LIFE SCIENCES

Description: Negative control for C2 ceramide.

UOM: 1 * 5 mg

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Catalog Number: (ENZOBMLSL1510005)

Supplier: ENZO LIFE SCIENCES

Description: Inhibits yeast growth and activates ceramide-activated protein phosphatase (CAPP).

UOM: 1 * 5 mg

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Supplier: ENZO LIFE SCIENCES

Description: Cell-permeable ceramide analog

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Supplier: Apollo Scientific

Description: A biologically active, cell permeable, non-physiological ceramide analog. It inhibits cell proliferation, induces monocytic differentiation of HL-60 cells & inducesapoptosis.

Catalog Number: (S6689-5MG)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: SynaptoRed™ C2 has been used for propidium iodide (PI) blockage.

UOM: 1 * 5 mg

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Supplier: Apollo Scientific

Description: (Boc-amino)-PEG3-C2-Carboxylic acid

Catalog Number: (ABCAAB77498-100)

Supplier: Abcam

Description: Anti-C2 Rabbit Polyclonal Antibody

UOM: 1 * 100 µG

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Catalog Number: (USBI124114)

Supplier: US Biological

Description: Anti-C2 Rabbit Polyclonal Antibody

UOM: 1 * 100 µG

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Catalog Number: (ABCAAB97461-50)

Supplier: Abcam

Description: Anti-C2 Rabbit Polyclonal Antibody

UOM: 1 * 50 µl

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Catalog Number: (USBI137569)

Supplier: US Biological

Description: Anti-C2 Goat Polyclonal Antibody

UOM: 1 * 1 mL

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