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PerfeCTa® qPCR ToughMix®

Supplier: Quantabio
Description: PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

MSDS Certificates

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Real Time PCR Detection Test Kits, Supreme HLVd/LCV/CanCv

Supplier: BIOPREMIER
Description: This Supreme RT-PCR detection kit HLVd/LCV/CanCV is designed for the detection of Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV) and Cannabis Cryptic Virus (CanCV) in a single PCR reaction (TETRAPLEX).

New Product

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PerfeCTa® qPCR FastMix™ reaction mixes

Supplier: Quantabio
Description: FastMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility, and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMixes is AccuFast™ <i>Taq</i> DNA polymerase that enables instant activation and rapid polymerisation kinetics. Upon heat activation (20 seconds at 95 °C), the antibodies irreversibly denature releasing fully active, unmodified <i>Taq</i> DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. FastMixes deliver maximum PCR efficiency, sensitivity, specificity, and a robust fluorescent signal with either fast or conventional cycling conditions.

MSDS

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PerfeCTa® QPCR mixes, SYBR® green super mixes and fast mixes

Supplier: Quantabio
Description: PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein.

MSDS Sale

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RNA isolation, E.Z.N.A.® HP total RNA kit

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and<i> in vitro</i> translation.

MSDS Certificates

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ReadiScript™ RT Reverse Transcription Kit

Catalog Number: (AATB60100)
Supplier: AAT BIOQUEST
Description: The two-tube kit format helps to set up the reaction faster and is optimised to yield cDNA over a broad dynamic range.
UOM: 1 * 50 Tests
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VWR®, Strips of PCR Tubes and Caps

Supplier: VWR Collection
Description: These PCR tube strips and caps are made of PP resin and are designed to fit most popular brands of thermal cyclers.

Environmentally Preferable

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RNA isolation, total RNA kit, E-Z 96®

Supplier: OMEGA BIO-TEK
Description: The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

MSDS

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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

New Product

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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

New Product

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TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

New Product

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TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

New Product

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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

New Product

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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

New Product

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TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

New Product

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TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

New Product

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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