You Searched For: G Bioscienes

Supplier: G-Biosciences

Description: Avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates. Avidin has a molecular weight of 67 kDa and contains four identical 128 amino acid subunits that each have a single biotin binding domain. This affinity purified avidin is ideal for estimation of biotin incorporation and other applications.

Catalog Number: (786-803)

Supplier: G-Biosciences

Description: The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

UOM: 1 * 1 KIT

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Catalog Number: (RC-095)

Supplier: G-Biosciences

Description: Disodium hydrogen phosphate

UOM: 1 * 500 g

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Catalog Number: (P319)

Supplier: G-Biosciences

Description: Potassium iodide

UOM: 1 * 100 g

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Catalog Number: (RC-089)

Supplier: G-Biosciences

Description: Sodium acetate

UOM: 1 * 500 g

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Supplier: G-Biosciences

Description: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Catalog Number: (RC-033)

Supplier: G-Biosciences

Description: Carbenicillin disodium salt

UOM: 1 * 1 g

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Supplier: G-Biosciences

Description: Polyethylene glycol 8,000

Supplier: G-Biosciences

Description: Tryptone is an assortment of peptides generated by the tryptic digestion of casein. It is commonly used in microbiology to produce Lysogeny broth for the growth of <i>E. coli</i> and other microorganisms, as it provides a source of amino acids.

Supplier: G-Biosciences

Description: D-(+)-Sucrose

Supplier: G-Biosciences

Description: Proteinase K (also protease K, endopeptidase K, peptidase K or Tritirachium alkaline phosphatase) (EC 3.4.21.64) is a non specifc, broad spectrum serine protease that is isolated from the saprophytic fungus <i>Tritirachium album</i>.

Supplier: G-Biosciences

Description: Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Catalog Number: (786-057)

Supplier: G-Biosciences

Description: ALLN is a cell permeable peptide aldehyde inhibitor of calpain I and to a lesser extent calpain II. It also inhibits other neutral cysteine proteases, cathepsin B and L and the proteasome.

UOM: 1 * 10 mg

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Catalog Number: (GENO786-422)

Supplier: G-Biosciences

Description: Acetate film with acrylic adhesive. This self-adhesive microplate sealing tape is suitable for sealing both 96-well and 384-well plates. The precut, pressure-senstive tape seals and reseals the surface of the plates during incubations to prevent evaporation and allow more vigorous mixing. The tape will reseal the plates 2 to 3 times.

UOM:

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Supplier: G-Biosciences

Description: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Catalog Number: (GENOBTNM-0079)

Supplier: G-Biosciences

Description: Part of the Ellyn Daugherty Biotechnology: Science for the New Millennium Program.

UOM: 1 * 500 g

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