You Searched For: G Bioscienes

Catalog Number: (786-129)

Supplier: G-Biosciences

Description: A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Catalog Number: (733-1945)

Supplier: G-Biosciences

Description: A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

UOM: 1 * 1 mL

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Supplier: G-Biosciences

Description: Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.

Catalog Number: (BE-404)

Supplier: G-Biosciences

Description: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Supplier: G-Biosciences

Description: Triton® X-114 is a non ionic detergent with a low cloud point (23 °C) making it suitable for protein solubilisation with phase-partitioning of hydrophilic proteins from amphiphilic proteins.

Supplier: G-Biosciences

Description: PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

Catalog Number: (786-431)

Supplier: G-Biosciences

Description: The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

UOM: 1 * 100 Assays

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Catalog Number: (RC-118)

Supplier: G-Biosciences

Description: Mineral oil, white (petroleum)

UOM: 1 * 500 mL

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Supplier: G-Biosciences

Description: IPTG is a molecular mimic of allolactose that initiates transcription of the lac operon.

Supplier: G-Biosciences

Description: Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Supplier: G-Biosciences

Description: G-Biosciences' Destain I contains approximately 45% methanol and 9% glacial acetic acid. G-Biosciences' Destain II contains approximately 5% methanol and 7% acetic acid.

Catalog Number: (786-529)

Supplier: G-Biosciences

Description: Imidazole buffer solution pH 7,5 (2 mol/l)

UOM: 1 * 1 L

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Supplier: G-Biosciences

Description: Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*. Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and it also binds non‐covalently to non‐polar regions in the protein.

Catalog Number: (786-683)

Supplier: G-Biosciences

Description: Blue-OUT™ for the removal of Coomassie and fluorescent stain prior to mass spectrometry

UOM: 1 * 1 KIT

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