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Supplier: G-Biosciences

Description: Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Supplier: G-Biosciences

Description: Triton® X-114 is a non ionic detergent with a low cloud point (23 °C) making it suitable for protein solubilisation with phase-partitioning of hydrophilic proteins from amphiphilic proteins.

Catalog Number: (BE-405)

Supplier: G-Biosciences

Description: This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.

UOM: 1 * 1 KIT

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Catalog Number: (BE-403)

Supplier: G-Biosciences

Description: Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

UOM: 1 * 1 KIT

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Catalog Number: (BE-414)

Supplier: G-Biosciences

Description: The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*. Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and it also binds non‐covalently to non‐polar regions in the protein.

Catalog Number: (786-683)

Supplier: G-Biosciences

Description: Blue-OUT™ for the removal of Coomassie and fluorescent stain prior to mass spectrometry

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Decontamination Solution, Endotoxin free water

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Supplier: G-Biosciences

Description: The Z-Competent™ <i>E. coli </i>transformation kit is designed to generate competent <i>E. coli</i> cells for simple and highly efficient <i>E. coli</i> transformation.

Catalog Number: (GD-6060)

Supplier: G-Biosciences

Description: HP Ampicillin™ is a stronger and more stable inhibitor of ß-lactamase than regular ampicillin and therefore supports only the growth of ampicillin resistant colonies even after prolonged incubation. Culture plates can be incubated for a longer period at 37 °C, eliminating the time constraint associated with normal ampicillin.

UOM: 1 * 5 items

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Catalog Number: (786-34)

Supplier: G-Biosciences

Description: BLOT-FastStain™ is a unique stain for reversible staining of protein on nitrocellulose and PVDF transfer membranes. It only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility.

UOM: 1 * 1 KIT

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Catalog Number: (733-1948)

Supplier: G-Biosciences

Description: A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

UOM: 1 * 1 mL

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Catalog Number: (786-397)

Supplier: G-Biosciences

Description: OmniPrep™ for Plant Kit isolates high quality genomic DNA from plant samples, including fresh and frozen plant tissues. If used according to the protocols this kit purifies DNA from 20gm plant tissue.

UOM: 1 * 1 KIT

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Catalog Number: (786-396)

Supplier: G-Biosciences

Description: OmniPrep™ for Blood Kit isolates high quality genomic DNA from blood samples, including whole blood, buffy coats, packed cells and bone marrow.

UOM: 1 * 1 KIT

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Catalog Number: (786-400)

Supplier: G-Biosciences

Description: OmniPrep™ for Yeast Kit isolates high quality genomic DNA from yeast. When used according to the recommended protocols, this kit purifies DNA from 300 ml yeast culture.

UOM: 1 * 1 KIT

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Catalog Number: (API-16S)

Supplier: G-Biosciences

Description: Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

UOM: 1 * 1 SET

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