You Searched For: G Bioscienes

Catalog Number: (BE-308)

Supplier: G-Biosciences

Description: Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

UOM: 1 * 1 KIT

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Catalog Number: (786-213)

Supplier: G-Biosciences

Description: CytoScan™-SRB Cytotoxicity Assay is based upon the quantitative staining of cellular proteins by sulforhodamine B (SRB). The assay is used for cell density determination, based on the measurement of cellular protein content.

UOM: 1 * 1.000 Assays

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Catalog Number: (BE-104)

Supplier: G-Biosciences

Description: This DNA fingerprinting kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo-crime scene.

UOM: 1 * 1 KIT

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Catalog Number: (786-499)

Supplier: G-Biosciences

Description: After Coomassie Brilliant Blue staining process, the band intensity may be further enhanced by de-staining the stained gel in our CBB De-Staining Solution.

UOM: 1 * 1 L

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Supplier: G-Biosciences

Description: Bisacrylamide, powder

Supplier: G-Biosciences

Description: FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.

Supplier: G-Biosciences

Description: Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Catalog Number: (RC-031)

Supplier: G-Biosciences

Description: Calcium chloride dihydrate

UOM: 1 * 1 kg

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Supplier: G-Biosciences

Description: PIPES monosodium salt

Supplier: G-Biosciences

Description: γ-(N-Morpholino)propanesulphonic acid (MOPS)

Supplier: G-Biosciences

Description: D-(-)-Leucine

Catalog Number: (RC-069)

Supplier: G-Biosciences

Description: Magnesium chloride

UOM: 1 * 500 g

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Supplier: G-Biosciences

Description: Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including <i>E.coli</i> strains.

Catalog Number: (BE-501)

Supplier: G-Biosciences

Description: This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Acrylamide (electrophoresis grade) is supplied as a powder or a 40% solution in ultrapure water. Bisacrylamide (Bis (N,N’-methylenebisacrylamide)) (electrophoresis grade) is supplied as a powder or a 2% solution in ultrapure water.

Supplier: G-Biosciences

Description: GET™ AGAROSE DNA method involves release of DNA fragments from gel pieces followed by capture of nucleic acids on the GET™ spin columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.