You Searched For: G Bioscienes

Catalog Number: (BE-414)

Supplier: G-Biosciences

Description: The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: G-Biosciences offers a wide selection of genomic isolation kits that purify high quality genomic DNA from a variety of sources and for a wide array of applications. G-Biosciences XIT™ DNA kits produce protein-free, high quality DNA through the principle of cell lysis, protein digestion and precipitation and genomic DNA purification. No chloroform or phenol extraction is required. High quality DNA can be isolated from sample types including: animal tissues, cells, whole blood, bacteria, buccal cells, plant tissues, mouse tail, yeast and FFPE tissue. G-Biosciences XIT™ DNA kit procedures remove contaminants and enzyme inhibitors allowing the purified DNA to be ready for immediate use for all downstream analyses. The purified DNA from G-Biosciences XIT™ DNA kits have a A₂₆₀/A₂₈₀ ratio between 1,7 and 1,9 (with the exception of the buccal cells kit with a ratio between 1,8 and 2,0) and are up to 200 kb in size.

Supplier: G-Biosciences

Description: Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*. Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and it also binds non‐covalently to non‐polar regions in the protein.

Catalog Number: (RC-118)

Supplier: G-Biosciences

Description: Mineral oil, white (petroleum)

UOM: 1 * 500 mL

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Supplier: G-Biosciences

Description: IPTG is a molecular mimic of allolactose that initiates transcription of the lac operon.

Supplier: G-Biosciences

Description: PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

Supplier: G-Biosciences

Description: The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Supplier: G-Biosciences

Description: Triton® X-114 is a non ionic detergent with a low cloud point (23 °C) making it suitable for protein solubilisation with phase-partitioning of hydrophilic proteins from amphiphilic proteins.

Catalog Number: (786-683)

Supplier: G-Biosciences

Description: Blue-OUT™ for the removal of Coomassie and fluorescent stain prior to mass spectrometry

UOM: 1 * 1 KIT

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Catalog Number: (BE-301)

Supplier: G-Biosciences

Description: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.

UOM: 1 * 1 KIT

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Catalog Number: (DG010)

Supplier: G-Biosciences

Description: TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether)

UOM: 1 * 10 items

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Supplier: G-Biosciences

Description: BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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Catalog Number: (786-R49)

Supplier: G-Biosciences

Description: Anti-IgG Rabbit Antibody (AP (Alkaline Phosphatase))

UOM: 1 * 1 mL

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Catalog Number: (BC17)

Supplier: G-Biosciences

Description: N-(ε-Maleimidocaproyloxy) sulfo succinimide ester Heterobifunctional Cross Linker.

UOM: 1 * 50 mg

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Catalog Number: (API-16S)

Supplier: G-Biosciences

Description: Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

UOM: 1 * 1 SET

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Supplier: G-Biosciences

Description: CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.